BASF Metabolome Solutions GmbH
Buildup of MetaMap® Tox database
MetaMap® Tox contains metabolome profiles of roughly 1100 compounds at different doses based on 28-day rat in vivo studies. In addition the database comprises more than 100 in vitro treatments of HepG2 cells.
Study design and buildup
The study design used to build up the database was based on a OECD 407 guideline with minor modifications. Blood was taken from Wistar rats (Crl:WI(Han)) treated with either a high dose (HD) or low dose (LD) of a test compound at days 7, 14 and 28 at the same time point every day.
For the buildup of the database, six mass spectrometric analyses were applied to the samples:
- gas chromatography mass spectrometry (GC-MS) and liquid chromatography MS/MS (LC-MS/MS) for broad profiling (both polar and lipid phase)
- solid phase extraction LC-MS/MS (SPE-LC-MS/MS) for determination of (a) steroid hormone and (b) catecholamine levels
